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AmJCaseRep

Estimation of the invasiveness of Helicobacter pylori strains on the basis of serological investigations in blood

Leokadia Bąk-Romaniszyn, Hanna Romanowicz, Stanisław Łukaszek, Andrzej Kulig, Magdalena Chmiela, Wiesława Rudnicka, Izabela Płaneta-Małecka

Med Sci Tech 2009; 50(3): RA137-140

ID: 881678

Available online:

Published: 2009-03-22


Introduction: Helicobacter pylori is a recognized factor inducing gastritis. Chronic gastritis with H.pylori infection is a precursor of chronic peptic ulcer disease as well as gastric cancer and lymphoma. Alterations in mucous membrane leading to the development of a neoplasm can last for years or even decades. The most pathogenic H.pylori strains are cagA+/vacA+ and infection with these strains causes particularly significant increase of gastric epithelial cells proliferation not accompanied by simultaneous intensification of apoptosis. The presence of antibodies against anti-H.pylori surface and specific antigens in blood of the infected subject corresponds to the infection with H.pylori strains of defined genotype. Aim: Estimation of the dependence between the occurrence and serum index of surface anti-H.pylori antibodies of IgG class, specific antibodies anti-CagA and the severity and activity of gastritis in the prepyloric part. Material and methods: The study comprised 154 children, aged 5–18 years (mean 13,6±3,6) with alimentary tract ailments. Basing on urease test, serological and histological findings two groups were distinguished: Group I – children with H.pylori infection (Hp+), N=99; Group II – children without current H.pylori infection (Hp-), N=55. ELISA was used to determine the serum level and index of anti-H.pylori antibodies of IgG class and anti-CagA antibodies. The presence, activity and severity of the inflammatory process were estimated in histological investigation of gastric mucosa specimens from prepyloric area. Pearson’s correlation or Spearman’s correlation coefficient was calculated to test the dependence. Results: Relatively high correlations were found between the value of the index of serum anti-H.pylori antibodies of IgG class and the occurrence of histological exponents of gastritis in prepyloric part (R=0,48, p<0,001). High positive correlation was emonstrated between the occurrence and index of anti-CagA antibodies in serum and the severity of gastritis in prepyloric part (respectively: R=0,51, p<0,001 and R=0,56, p<0,001) and the activity of the inflammation (R=0,48, p<0,001). Conclusion: There is a strong correlation between the level of anti-H.pylori antibodies of IgG class, the presence and the level of anti-CagA H.pylori antibodies in serum and the severity and activity of gastritis in prepyloric part. (Clin Exp Med Lett 2009; 50(3):137-140)

Keywords: Helicobacter pylori



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