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AmJCaseRep

Effects of nandrolone decanoate on activity of matrix metalloproteinase 2 in tibialis anterior muscle during the repair process

Agnelo Neves Alves, Kristianne Porta Santos Fernandes, Camila Aparecida Alves da Silva, Alessandro Melo Deana, Cristiane Miranda França, Sandra Kalil Bussadori, Fabio Daumas Nunes, Raquel Agnelli Mesquita Ferrari

Med Sci Tech 2012; 53(2): RA75-79

ID: 883578

Available online:

Published: 2012-11-19


#883578

Background:    Nandrolone decanoate (ND) is an anabolic steroid that is widely used by professional and amateur athletes who frequently experience muscle injury. The matrix metalloproteinase 2 (MMP-2) plays a key role in the migration, differentiation and fusion of satellite cells, as well as facilitating the recruitment of inflammatory, vascular and fibroblast cells to damaged tissue. The aim of the present study was to analyze the effect of ND on MMP-2 activity during the repair process of tibialis anterior muscle.
    Material/Methods:    Fifty Wistar rats were divided into 4 groups: 1) control; 2) sham; 3) untreated injury; and (4) injury + treatment with ND. Cryoinjury was performed on the belly of the tibialis anterior (TA) muscle. Treatment consisted of subcutaneous injections of ND (5 mg/kg) on the back of the animal twice a week. The groups were assessed at 1, 7, 14 and 21 days. At the end of the protocol, the animals were sacrificed and the TA muscles were removed for the analysis of MMP-2 activity and characterization using zymography and Western blotting.
    Results:    ND induced an increase in MMP-2 activity on Days 1 and 14 following injury, and significantly reduced this activity on Days 7 and 21 in comparison to the untreated injury group.
    Conclusions:    These findings suggest that the anabolic steroid ND was able to modulate MMP-2 activity during the muscle repair process in a time-dependent manner, causing an increase in activity after 1 and 14 days, and a decrease after 7 and 21 days.

Keywords: skeletal muscle, Nandrolone Decanoate, Matrix Metalloproteinase 2, injury



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