Biochemical effects of ghrelin on carbon tetrachloride-induced experimental acute liver injury in rats
Sabiha Sahin, Ozkan Alatas
Med Sci Tech 2010; 51(3-4): RA175-179
Background: Ghrelin is a peptide that has protective effects on many tissues of the body, and it has anti-inflammatory and anti-oxidant effects. Antioxidant properties of ghrelin on various oxidative stresses in limited tissues have been recently reported. Therefore, we designed this study to explore the probable antioxidative effects of ghrelin on acute liver injury induced by carbon tetrachloride in rats.
Material/Methods: In this experimental study, 24 Sprague-Dawley albino rats were equally divided into 3 groups as follows: control, CCl4, and CCl4+Ghrelin. Four ml/kg olive oil was administered intraperitoneally (i.p.) to the control group, and 4 ml/kg CCl4 (1.1 dissolved in olive oil) was administered i.p. to the animals in the other 2 groups. After 3 and 6 hours, 80 mcg/kg ghrelin was administered i.p. to the CCl4+Ghrelin group. Twenty-four hours after administrating CCl4, all of the rats were sacrificed. Biochemical assessments were performed using serum AST, ALT, MDA (Malondialdehyde), tissue MDA, MPO (Myeloperoxidase) and NO (nitric oxide) levels.
Results: Serum AST, ALT, MDA and tissue MDA, MPO levels all increased in the CCl4 group, but they were significantly decreased in the group treated with ghrelin. Tissue NO levels decreased in the CCl4 group, but there was a more limited decrease in the group treated with ghrelin.
Conclusions: Acute liver injury can result from several factors, and there are no effective therapies for patients with acute liver injury. Our study supports that ghrelin protected rats from acute liver injury by reducing oxidative stress and inflammation.
Keywords: ghrelin,, CCl4 , acute hepatic injury