Evaluation of relations between serum homocysteine concentration and activity of the selected parameters of lipid levels, antioxidant barrier and expression of adhesion molecules in children with atherosclerosis risk factors
Anna Sierakowska-Fijałek, Zbigniew Baj, Mariusz Stępień, Jacek Rysz
Med Sci Tech 2010; 51(3-4): RA209-215
Background: The aim of this study was to explore the relationship between serum homocysteine concentration and activity of chosen parameters of lipid levels, activity of the selected parameters of the antioxidant barrier, and expression of the adhesion molecules in children with atherosclerosis risk factors.
Material/Methods: The study screened 670 children. We determined total cholesterol (TC), triglycerides (TG), LDL cholesterol fraction (LDL-C), HDL cholesterol fraction (HDL-C), serum homocysteine concentration (Hcy), the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), malonyl dialdehyde concentration (MDA), and expression of the soluble forms of adhesion molecules (sCAM): sP-selectine and sVCAM-1 (vascular cell adhesion molecules-1).
Results: Obesity, hypertension and lipid disorders in the form of higher concentrations of TC, LDL-C, TG, and lower HDL-C were the most frequent risk factors in the study group children. No significant differences between groups were observed in serum homocysteine concentrations; however, serum homocysteine concentration was significantly higher in children with 2 atherosclerosis risk factors. Activity of GSH-Px was statistically significantly higher in children with atherosclerosis risk factor, activity of CAT was lower compared to the control group. No significant differences in activity of SOD and MDA concentrations were observed in the investigated groups.Statistically significantly higher serum concentration of lipid levels and lower activity of catalase were found in children with hyperhomocysteinemia compared with controls. No significant differences in expression of sVCAM-1 were observed in the investigated groups, and concentration of sP-selectine was significantly higher in children with atherosclerosis risk factors.
Conclusions: Higher serum homocysteine concentration, selected adhesion molecules and disorders of the antioxidant barrier in children with atherosclerosis risk factors are potential markers of risk for early development of atherosclerosis.
Keywords: lipid level, homocysteine, children, atherosclerosis, Antioxidants, adhesion molecules