Effect of vasodilators on lipid peroxidation and antioxidative mechanisms in gastric mucosa
Sławomir Kwiecień, Michał W Pawlik, Iwona Brzozowska, Zbigniew Śliwowski, Tomasz Brzozowski, Wiesław W Pawlik, Nina Kwiecień, Stanisław J Konturek
Med Sci Tech 2005; 46(3): RA33-38
ID: 881469
Available online:
Published: 1999-11-30
Acute gastric mucosal lesions make an important clinical problem. The important role in maintenance of gastric barrier integrity plays nitric oxide (NO). NO has been an aim of many scientific researches. However the role of reactive oxygenspecies (ROS) in interaction between NO and gastric mucosa has been little studied. The purpose of our present investigations was to explain the participation of ROS in healing of stress-induced gastric ulcers accelerated by NO. Experiments were carrying out on Wistar rats. We demonstrated that 3.5 h of water immersion and restraint stress (WRS) resulted in appearance of acute gastric mucosal lesions accompanied by a decrease of GBF. Biological effects of ROS were estimated by measuring tissue level of MDA and 4-HNE, the products of lipid peroxidation by ROS as well as the SOD activity, the scavanger of ROS. It was demonstrated that WRS led to significant increase of MDAand4-HNE mucosal level, accompanied by a decrease of SOD activity. Pretreatment with NO-donors (SIN-1, SNAP, nitroglycerin, NO-ASA) resulted in reduction of gastric lesions number, increment of GBF, decrease of MDA and 4-HNE tissue level and increase of SOD activity. ROS play a role in NO-donors action in healing of gastric acute lesions induced by WRS. NO-donors cause attenuation of lipid peroxidation what indicates a decrease of MDA and 4-HNE levels as well as NO-donors enhance antioxidative properties of cells measured by increase of SOD activity. (Clin. Exp. Med. Lett. 2005; 46(3):33-38)
Keywords: Gastric Mucosa, MDA, SOD, NO, SIN-1, SNAP, Nitroglycerin, NO-aspirin