e-ISSN 2329-0072

Logo

Medical
MSM  BR

AmJCaseRep

Get your full text copy in PDF

Post-treatment with ambroxol and N-acetylcysteine reduces the doxorubicin-induced oxidative stress in hearts and lungs of mice

Piotr Czech, Marek Kasielski, Marek Mokwiński, Arkadiusz Balcerowski, Piotr Białasiewicz, Dariusz Nowak

Med Sci Tech 2006; 47(3): RA161-167

ID: 881509


Introduction: Ambroxol and N-acetylcysteine are widely used mucolytic drugs that have antioxidant properties. We tested whether these drugs at the iv. dose of 0.169 mmoles/kg of body weight can inhibit the doxorubicin (DOX)-induced oxidative stress in murine heart and lungs. Material and Methods: Mice (n=10 in each group) were injected iv. with DOX (30 mg/kg) or 0.9% NaCl and then after 30 min with ambroxol, N-acetylcysteine, the combination of both drugs, or 0.9% NaCl. At 5th h from DOX injection the concentration of thiobarbituric acid reactive substances (TBARs) and Schiff bases (SB)- two end-products of lipidperoxidation, and the activity of H2O2 were determined in heart and lung homogenates. Results: Ambroxol and N-acetylcysteine significantly attenuated the DOX-elicited rise in the end-products of lipid peroxidation and H2O2 activity in hearts and lungs. Post-treatment with the combination of ambroxol and N-acetylcysteine was the most effective on inhibition of DOX-induced TBARs and H2O2 generation in heart homogenates. The heart levels of TBARs and H2O2 decreased from 2.73±0.15 μM/g and 6.02±0.41 μM in mice injected with DOX and 0.9% NaCl to 1.55±0.09 μM/g and 0.57± 0.04 μM in animals treated with DOX and ambroxol plus N-acetylcysteine (p<0.05), respectively. However, the inhibitory effect on DOX-evoked SB formation in hearts and lungs was similar for all antioxidant treatments. Conclusions: These indicate that ambroxoland N-acetylcysteine can inhibitoxidative stress in mice treated with single injection of DOX. Combination of ambroxol and N-acetylcysteine seems to be a possible candidate for prevention of the anthracycline-induced cardiotoxicity. (Clin. Exp. Med. Lett. 2006; 47(3):161-167)

Keywords: Ambroxol, N-acetylcysteine, heart injury

This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
I agree