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Detecting and quantifying absorbed irrigation fluid by photometric/enzymatic measuring sorbitol concentrations in serum samples

Anton Oertl, Roman Blaheta, Dietger Jonas, Gerhard M Oremek

Med Sci Tech 2006; 47(4): RA209-213

ID: 881524

Introduction: Endoscopic procedures of prostate, bladder and endometrium resection are established since many years. The influx of the irrigation fluid can be detected in different ways. The knowledge of the absorption level is necessary to prevent patients from threatening TUR syndrome. Previous detection methods were performed by additional indicators (e.g. ethanol) or by direct detection of ingredients in the irrigation fluid with a high apparative and time effort. Material and methods: This study shows the correlation between concentration and enzymatic detection of sorbitol. In clinical part of the study the influxrates between 0 land 2.7 l of 10 patients from our patient group after transurethral resection of the prostatic gland were calculated. Theme asurements were done during the obligatory pre-and postoperative control of the serum sodium level under threefold control measurements. Results: The calculation of sorbitol influx was expressed by the substraction of extinction of the samples gathered before the operation from the corresponding samples from the patients after the operation and converted to the absolutes orbitolconcentration by means of the calibration curve. A significant increase of ΔS - Na is found in the high influx range. In contrast, the ΔS-Na values are not realisable in the range of influx rates smaller than 1l. Mostly only intraoperative course and detection of high difference between preoperative and postoperative serum sodium levels are indicators for a high significant influx. Conclusions: The photometric/enzymatic detection of sorbitol in human plasma is well suitable in clinical routine diagnostics to measure the influx of irrigation fluid in subclinical ranges due to its reliability and relatively small apparative efforts.(Clin. Exp. Med. Lett. 2006; 47(4):209-213)

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