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Cellular inflammatory response influenced by Helicobacter pylori in children with the infection

Elżbieta Czkwianianc, Wiesława Rudnicka, Magdalena Chmiela, Izabela Płaneta-Małecka, Ewa Małecka-Panas

Med Sci Tech 2009; 50(3): RA141-147

ID: 881680


Introduction: Initial infection with H.pylori usually occurs early in childhood and unless treated, persists throughout life in most individuals. The mechanism related to the persistence of H.pylori remains unclear. Despite the host generates a huge, local and general inflammatory response to this infection; the immune system usually fails to eradicate the bacteria. Aim: The study was aimed to investigate cellular response to H.pylori in children. Material and methods: 48 consecutive chronically dyspeptic children aged 6-17y underwent upper GI endoscopy with gastric biopsy. Endoscopy lesions and pathology scoring with upgraded Sydney System in the biopsies were estimated as well as H.pylori infection status. Blood samples from examined children were taken to determine sera IgG-antibodies and to obtain peripheral blood mononuclear leucocytes (PBML). PBML proliferation was examined after stimulation with H.pylori heated rods, lipopolysaccharide, glycine extract and with PHA (control of the reactions), used alone and in the interleukin-2 (IL2) milieu. Results: 20 of the examined children were Hp. infected and 18 were not. The mean PBML proliferation value from infected children responded to H.pylori rods amounted to 2081.1±1606.3 cpm/culture and it rose to 4573.4±2277.9 cpm/culture after IL-2 addition and was significantly weaker (3514.2±2178.6 cpm/culture and 6985.5±3798.2 cpm/culture, respectively) then in the control group (p<0.05). A significant statistically correlation between the PMBL proliferation and gastric glands atrophy in the infected children was seen (r=0.611 p<0.05). Conclusions: H.pylori has weakened the cellular response to the infection what may favor the long lasting gastric colonization and the bacterial induced gastric epithelial and mucosal damage. (Clin Exp Med Lett 2009; 50(3):141-147)

Keywords: Helicobacter pylori, cellular immunity, children, chronic dyspepsia

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