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Low-level laser therapy reduces edema, leukocyte influx and hyperalgesia induced by Bothrops jararacussu snake venom

Ludmila Guimarães Souza, Camila Squarzoni Dale, Nikele Nadur-Andrade, Ana Maria Barbosa, José Carlos Cogo, Stella Regina Zamuner

Med Sci Tech 2011; 52(3-4): RA97-102

ID: 882238


Background:    Inflammation and pain are the most pronounced local effects caused by B. jararacussu snake venom. AV therapy and other first-aid treatments do not reverse these local effects. Thus, there is an urgent need to find therapies that can complement antivenoms in the neutralization of local tissue damage. The present work was designed to investigate the effect of Low-Level Laser Therapy (LLLT) on inflammatory parameters and hyperalgesia induced by B. jararacussu venom (BjssuV).
    Material/Methods:    A total of 50 Swiss mice were used for the study. The mice were distributed in 3 equal groups; a control group injected with saline, a venom-injected group, and a group injected with venom and treated with a laser. Paw edema was evaluated by plethysmometry. Tactile allodynia and mechanical hyperalgesia were evaluated by von Frey filaments. Leukocyte influx in the peritoneal cavity was determined by total and differential cell counts. The treated group was irradiated with an AsGa laser (685 nm, 30 mW and a 0.2 cm2 beam) applied transcutaneously to a single point with an energy density of 2.2 J/cm2.
    Results:    BjssuV induced significant edema, tactile allodynia and mechanical hyperalgesia in the mice paw. Prominent leukocyte infiltrate into the peritoneum was also observed. LLLT significantly reduced all evaluated parameters.
    Conclusions:    LLLT significantly reduced the effects of BjssuV on the inflammatory and hyperalgesia response. Therefore LLLT should be considered as a potential therapeutical approach for treatment of local effects of Bothrops envenomation.

Keywords: Hyperalgesia, myotoxins, lasertherapy, B. jararacussu, Edema

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