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Effect of therapeutic pulsed ultrasound on the proliferation and gelatinolytic activity of myoblasts

Rafael Ribeiro, Kristianne Porta Santos Fernandes, Manoela Domingues Martins, Sandra Kalil Bussadori, Crislene Viscardi Sampaio, Raquel Agnelli Mesquita-Ferrari

Med Sci Tech 2012; 53(3): RA107-112

ID: 883608

Background:    The muscle regeneration process involves inflammation, regeneration, and remodeling, in which the muscle extracellular matrix is mainly performed by matrix metalloproteinases (MMPs). In skeletal muscle, MMP-2 and MMP-9 have been shown to be involved in response to injury, as well as myopathic- and inflammatory-induced alterations. Ultrasound is often used as a therapy for muscular repair; however, there is insufficient evidence to provide a scientific foundation regarding its effectiveness in muscle regeneration. This study evaluated the effect of ultrasound on the proliferation and remodeling of satellite cells.
    Material/Methods:    C2C12 myoblasts were irradiated with ultrasound in pulsed mode at 20%, 1 and 3 MHz, intensities of 0.2 and 0.5 W/cm2 for 2 and 5 minutes. Cell proliferation was assessed 72 hours after irradiation and proteinase activity was evaluated by zymography and densitometry analysis.
    Results:    There were no significant differences in cell proliferation and densitometry analysis between ultrasound-treated myoblasts and control cultures.
    Conclusions:    Ultrasound did not alter C2C12 proliferation, viability, or proteinase activity.

Keywords: Low-intenstiy ultrasound, rehabilitation, Muscle Cells, C2C12 cells, Extracellular Matrix

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