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Rafael Ribeiro, Kristianne Porta Santos Fernandes, Manoela Domingues Martins, Sandra Kalil Bussadori, Crislene Viscardi Sampaio, Raquel Agnelli Mesquita-Ferrari
Med Sci Tech 2012; 53(3): RA107-112
ID: 883608
Background: The muscle regeneration process involves inflammation, regeneration, and remodeling, in which the muscle extracellular matrix is mainly performed by matrix metalloproteinases (MMPs). In skeletal muscle, MMP-2 and MMP-9 have been shown to be involved in response to injury, as well as myopathic- and inflammatory-induced alterations. Ultrasound is often used as a therapy for muscular repair; however, there is insufficient evidence to provide a scientific foundation regarding its effectiveness in muscle regeneration. This study evaluated the effect of ultrasound on the proliferation and remodeling of satellite cells.
Material/Methods: C2C12 myoblasts were irradiated with ultrasound in pulsed mode at 20%, 1 and 3 MHz, intensities of 0.2 and 0.5 W/cm2 for 2 and 5 minutes. Cell proliferation was assessed 72 hours after irradiation and proteinase activity was evaluated by zymography and densitometry analysis.
Results: There were no significant differences in cell proliferation and densitometry analysis between ultrasound-treated myoblasts and control cultures.
Conclusions: Ultrasound did not alter C2C12 proliferation, viability, or proteinase activity.
Keywords: Low-intenstiy ultrasound, rehabilitation, Muscle Cells, C2C12 cells, Extracellular Matrix